Maximum Likelihood phylogenetic tree of selected samples of Pristimantis, representing the P. lacrimosus species group and related taxa, inferred from an alignment of 1637 nucleotides of the mitochondrial 16S rRNA gene. Three samples of Yunganastes were used to root the tree (not shown for better graphical presentation). Numbers at nodes are bootstrap values in percent (2000 pseudoreplicates; not shown if <50%), followed by SH-aLRT values (1000 replicates; not shown if <50) as calculated with IQ-TREE. The taxon name is followed by the GenBank accession number, or voucher number for newly produced sequences (bold terminals), and the sample locality. Inset photo depicts the holotype of P. loeslein sp. nov. (MUSM 40302) in life (mirrored).