Gene;"Initial denaturation";"Cycles (denaturation / annealing / extension)";"Final extension";Reference
"cyt b";"3 min at 94°C";"40 cycles (45 sec at 94°C / 45 sec at 50->45°C (touchdown) / 1.5 min at 72°C)";"5 min at 72°C";"based on Weyeneth et al. (2008)"
COI;"1 min at 94°C";"5 cycles (30 sec at 94°C / 40 sec at 50°C / 1 min at 72°C), followed by 35 cycles (30 sec at 94°C / 40 sec at 55°C / 1 min at 72°C)";"10 min at 72°C";"De Pasquale and Galimberti (2014)"
Rag2;"3 min at 94°C";"39 cycles (45 sec at 94°C, 45 sec at 60°C and 1.5 min at 72°C)";"5 min at 72°C";"Stadelmann et al. (2007)"
"ABHD11 & THY";"2 min at 94°C";"2-2 cycles (15 sec at 95°C / 30 sec at 65, 63, 61, 59, and 57°C (touchdown) / 1 min at 72°C), followed by 30 cycles (15 sec at 95°C / 30 sec at 55°C / 1 min at 72°C)";"5 min at 72°C";"Dool et al. (2016)"
PRKC1;"3 min at 94°C";"39 cycles (30 sec at 94°C / 1.5 min at 53°C / 1 min at 72°C)";"10 min at 72°C";"Eick et al. (2005)"