Bioacoustic variables that significantly differentiate the Leptodactylus fuscus clades based on Kruskal-Wallis and Dunn tests. A Call duration between advertisement calls of males of the L. fuscus clade 1 and L. fuscus clade 2; B low frequency between advertisement calls of males of the L. fuscus clade 1 and L. fuscus clade 2; C delta frequency between advertisement calls of males of the L. fuscus clade 2 and L. fuscus clade 3; D high frequency between advertisement calls of males of the L. fuscus clade 2 and L. fuscus clade 3; E 95% frequency between advertisement calls of males of the L. fuscus clade 2 and L. fuscus clade 3; F dominant frequency between advertisement calls of males of the L. fuscus clade 2 and L. fuscus clade 3. Boxplots denote median, first and third quartiles, and outliers. S = seconds, Hz = Hertz.