The sampling localities of all Thryonomys swinderianus specimens successfully sequenced in the current study. The green triangle shows the location of the new specimen found outside the known distribution from the Eastern Cape Province. Museum specimens also observed outside the accepted distribution range can be seen in KwaZulu-Natal, North-West and Gauteng Provinces (KZN, NW and GP). The shaded area represents the currently accepted distribution range as obtained from the IUCN (Child 2016). The South African provincial borders are also shown. The Great Fish River system (pink) and Orange River system (orange) are also depicted on the map.