We describe a new species of Pristimantis from lowland Amazonia of the Region Loreto, northern Peru. The new species is mainly characterized by its size (SVL 26.5 mm in male, 33.0 mm in female), shagreen dorsal skin, absence of dorsolateral folds, slightly areolate venter, presence of discoidal fold, presence of tympanic membrane including tympanic annulus, absence of vocal slits in males, presence of ulnar and tarsal tubercles, bright green to yellow-green dorsal coloration with irregular pattern of small white spots, brown loreal-tympanic region, brown stripe on flanks sharply outlining dorsal coloration, white to yellow throat, yellow belly, and dark brown palmar and plantar surfaces. The new species is tentatively assigned to the Pristimantis unistrigatus Group. It has an arboreal mode of life and inhabits primary and disturbed Amazonian lowland rainforest.

Amphibia, Anura, Strabomantidae, Pristimantis padiali, new species, Amazonian lowlands, Peru.