Corresponding author: Pablo J. Venegas ( pvenegas@corbidi.org ) Academic editor: Raffael Ernst
© 2021 Pablo J. Venegas, Luis A. García-Ayachi, Lourdes Y. Echevarría, Daniel J. Paluh, Juan C. Chávez–Arribasplata, Axel Marchelie, Alessandro Catenazzi.
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation:
Venegas PJ, García-Ayachi LA, Echevarría LY, Paluh DJ, Chávez–Arribasplata JC, Marchelie A, Catenazzi A (2021) A new species of marsupial frog (Anura; Gastrotheca) from the Cordillera de Colán in northeastern Peru. Vertebrate Zoology 71: 201-218. https://doi.org/10.3897/vz.71.e60097
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We describe a new species of marsupial frog, genus Gastrotheca, using morphological characters and molecular data as lines of evidence. The new species was discovered in the páramo and the ecotone between páramo and humid montane forest of Cordillera de Colán, at elevations between 3136 and 3179 m a.s.l., in northeastern Peru. The new species is distinguished from all its congeners by the combination of the following characters: coarsely granular skin on dorsum, a green dorsal coloration without pattern, finger I shorter than finger II, turquoise iris, and a venter without blotches, flecks or dots. Furthermore, we include a detailed osteological description of the new Gastrotheca species based on Micro-CT scanning. Based on our phylogenetic analyses, the new species belongs to the Gastrotheca marsupiata species group, is sister to G. oresbios and closely related to G. psychrophila, G. spectabilis, G. stictopleura and one undescribed species. Additionally, we test for the presence of the fungal pathogen Batrachochytrium dendrobatidis (Bd). No Bd infection was detected for G. gemma sp. nov. specimens but Bd prevalence was detected among syntopic frogs.
Amphibians, Andes, Batrachochytrium dendrobatidis, Gastrotheca abdita, Hemiphractidae, osteology, phylogeny
With 75 described species, the genus Gastrotheca—commonly known as marsupial frogs because females brood eggs in closed dorsal pouches (
Among the thirty species of Gastrotheca that occur in Peru (
The Cordillera de Colán is a mountain ridge of moderate elevation in the Andes (< 3700 m a.s.l.), isolated from the main chain of the Andes by the Chiriaco and Utcubamba rivers that flow northward into the upper Marañón River, the main tributary of the Amazon River (
The amphibian disease chytridiomycosis has decimated frog communities in Andean montane forests and grasslands (
The first author (PJV) conducted two herpetological surveys to the Cordillera de Colán in 2019, and discovered several putative new species of amphibians and reptiles inhabiting the montane forest and páramos of these remote mountains. Herein, we describe a new species of marsupial frog collected in a narrow páramo at the top of this poorly known mountain ridge. We integrate several lines of evidence, including a detailed osteological examination using computed tomography, external meristic and morphological traits, and molecular data to characterize and diagnose the new species. Finally, in recognition of the threat of chytridiomycosis for montane forest and páramo amphibians in the Andes, we quantified pathogen prevalence and intensity of infection in the new species and syntopic frogs.
We surveyed frogs along existing trails and new open trails during five days, and captured frogs by hand. For each capture, we recorded coordinates and elevation with a GPS (Garmin, WGS84), and we swabbed the skin for detection of the fungal pathogen Batrachochytrium dendrobatidis. This procedure does not harm the frogs (
External morphological characters and format of description follow
We scanned the brooding female paratype of Gastrotheca gemma sp. nov. (
We performed phylogenetic analyses in order to infer the phylogenetic position of Gastrotheca gemma sp. nov. within the genus. We used muscle tissue of the holotype (
We downloaded sequences of the following genes from GenBank DNA: 12S rRNA, 16S rRNA, NADH dehydrogenase subunit 1 (ND1), proopiomelanocortin A (POMC) and the recombination activating gene 1 (RAG1). We included terminals from all supraspecific clades currently considered for Gastrotheca (
We aligned sequences of each gene in Aliview 1.17.1 (
To describe the results, we follow the taxonomy proposed by
We considered multiple lines of evidence to support delimitation of the new species. For molecular analyses, we consider a species as the single lineage segment of ancestor-descendant populations or metapopulations evolving separately from other lineages (
We used a real-time Polymerase Chain Reaction (PCR) assay on DNA material collected on skin swabs to quantify the level of Bd infection (
The molecular dataset includes 68 terminals, 59 of which represent described species of Gastrotheca, and 4,919 aligned base pairs. According to the PartitionFinder results, we considered the five loci independently and applied the GTR + I + G model to each. The maximum likelihood optimal tree has a log likelihood score of –36764.630331 (Fig.
The Gastrotheca fissipes, G. longipes, G. marsupiata and G. microdiscus species groups were recovered as monophyletic. Only one terminal of the G. walkeri species group was included. Gastrotheca gemma sp. nov. was recovered within the Gastrotheca marsupiata species group, as sister to G. oresbios. Other closely related species to G. gemma are: G. psychrophila, G. spectabilis, G. stictopleura and one undescribed species. The matrix of uncorrected p-distances for a 16S rRNA fragment shows that G. oresbios and G. spectabilis had the lowest distances with respect to G. gemma (Table
Maximum likelihood optimal tree (log likelihood = –36764.630331) inferred from 4,919 bp of mitochondrial (12S rRNA, 16S rRNA, ND1) and nuclear (POMC and RAG1) gene sequences. The phylogenetic tree depicts the relationships of Gastrotheca gemma sp. nov. with 67 Gastrotheca terminals, including 59 described species. Bootstrap support values of 100% are represented by an asterisk. Species groups are abbreviated as s.g.
Uncorrected p-distances of the mitochondrial 16S rRNA gene between Gastrotheca gemma sp. nov. and closely related Gastrotheca species.
1 | 2 | 3 | 4 | 5 | |
1. G. gemma |
|||||
2. G. oresbios |
2.3 | ||||
3. G. psychrophila KU 142634 | 5.2 | 5.2 | |||
4. G. spectabilis |
4.5 | 4.8 | 4.3 | ||
5. G. stictopleura MTD 45230 | 5.2 | 5.4 | 5.2 | 5.0 | |
6. G. sp. |
5.2 | 5.7 | 5.4 | 6.6 | 5.4 |
PERU • 1 ♀, a brooding adult; Amazonas department, Utcubamba province, Cajaruro district, from the trail from Refugio Lechucita to El Hito; 5°36’58.7’’S, 78°14’58.8’’W; 3180 m a.s.l.; 25 Nov. 2019; P.J. Venegas, L.A. García-Ayachi, J.C. Chávez-Arribasplata, J.R. Ormeño, S. Bullard and A. Marchelie leg.;
PERU • 1 ♀, adult; Amazonas department, Utcubamba province, Cajaruro district, from El Hito; 5°36’45.3’’S, 78°15’2.9’’W; 3300 m a.s.l.; 09 Nov. 2017; A. García-Bravo leg.;
Assigned to the genus Gastrotheca by females possessing a closed brood pouch on the dorsum. A moderately large species (69.7 and 71.8 mm SVL in two females, 56.9 and 59.5 mm SVL in two males), with: (1) tibia length 57–59% SVL, longer than foot; (2) interorbital distance greater than width of upper eyelid (161–169%); (3) skin on dorsum coarsely granular in females and granular in males, not co-ossified with skull, lacking transverse ridges; (4) supraciliary processes absent; (5) heel lacking calcar or tubercle; (6) tympanic annulus wrinkled or tuberculate; (7) Finger I slightly shorter than Finger II, width of discs wider than digits; (8) finger webbing present basally, only between III and IV; (9) foot webbing between external toes extending to nearly antepenultimate subarticular tubercle on Toe IV, to penultimate subarticular tubercle on Toe V; (10) in life, dorsum green with numerous minute black flecks in females and green with scattered yellow dots in males, paravertebral marks absent; (11) head markings consisting of a chocolate or pale green labial stripe in females and males, respectively; (12) dorsolateral stripe absent; (13) flanks uniformly green in females and green with numerous dark green irregular flecks in males; groin yellowish green in both sexes; anterior surfaces of thighs green, posterior surfaces of thighs yellowish green with scattered irregular black flecks in females and dense black reticulations in males; ventrolateral region yellowish green; irises silvery with a light blue hue or turquoise with thin black reticulations with or without an orange ring; (14) gular region and chest green in females and yellowish green in males; venter yellowish green with or without a big dark grey patch in the middle in females and venter greenish cream in males; ventral surface of thighs yellowish green with a dark greyish brown patch on the centre in females and thighs pale brownish cream in males; palms, soles and ventral surface of tarsus dark grey or dark greyish brown; (15) brood pouch single, dorsal; (16) reproduction mode direct development.
Gastrotheca gemma closely resembles G. aguaruna, G. carinaceps, and G. dysprosita from Peru, and G. turnerorum from Ecuador. Gastrotheca gemma shares with the aforementioned species a granular dorsal skin and a green dorsum, however the skin texture in G. gemma is coarsely granular, whereas it is weakly or finely granular in G. aguaruna and G. carinaceps, and granular in G. turnerorum. Furthermore, G. aguaruna differs by having finger I longer than II, and G. carinaceps finger I and II equal in size (finger I shorter than finger II in G. gemma). Gastrotheca aguaruna has a dorsolateral row of warts or tubercles, absent in G. gemma. Gastrotheca turnerorum also can be distinguished from G. gemma by having brown flanks and a dark brown venter with cream spots, while G. gemma has yellowish green flanks. The new species and G. dysprosita have a coarsely granular dorsum, however G. dysprosita differs from G. gemma (character in parentheses) by having the interorbital distance smaller than width of upper eyelid (interorbital distance greater than width of upper eyelid), finger I and finger II equal in size (finger I shorter than finger II), dorsum with middorsal and dorsolateral stripes (dorsum without pattern), and venter cream with small brown spots (spots or blotches absent).
Gastrotheca gemma can be confused with green individuals of G. monticola and G. ossilaginis that also occur in the Andes of northern Peru (
Phylogenetically, G. gemma is sister to G. oresbios and closely related to G. psychrophila, G. spectabilis and G. stictopleura. The skin on the dorsum of the adult female holotype of G. oresbios, the single known adult specimen of this species, was described as smooth with scattered tubercles (see
Gastrotheca gemma was found in syntopy with G. abdita in the páramo, however both can be easily distinguished by their skin texture, being smooth in G. abdita. Furthermore, the dorsum is usually brown with paravertebral stripes in G. abdita, but green in the new species. Gastrotheca testudinea also occurs in Cordillera de Colán but at lower elevations (from 1700 to 2200 m a.s.l.). Gastrotheca testudinea differs from the new species by having the dorsum smooth and finger I longer than finger II. Additionally, as in G. abdita, the dorsum of G. testudinea is usually brown.
An adult female (Fig.
Arm robust; ulnar tubercles absent; hand and fingers moderately large (TFL 38 % of SVL); fingers with basal web only between III and IV; discs large and rounded, width of disc of Finger III greater that diameter of tympanum; relative lengths of fingers I<II<IV<III; subarticular tubercles prominent, round in dorsal and profile views, none bifid; supernumerary tubercles, round; palmar tubercle ill-defined, bifid; prepollical tubercle large, elliptical. Hind limb robust; tibia length 59% of SVL; foot length 54% of SVL; calcar and tarsal tubercles absent; inner tarsal fold present; outer metatarsal tubercle absent; inner metatarsal tubercle elliptical, low; toes moderately long; relative length of toes I<II<III<V<IV; basal webbing between Toes I and II; webbing formula for other toes II1–2III1–2½IV2–1V; subarticular tubercles moderately large, rounded; supernumerary tubercles, numerous, and rounded; outer edge of toe V bears a fringe extending along the edge of foot.
Skin on dorsum coarsely granular; skin on flanks covered by enlarged flattened warts; skin on throat and chest weakly granular, ventral surfaces of thighs and arms coarsely granular; skin on belly granular; ventral surface of shanks smooth; two vertical rows of enlarged brown tubercles (three on left and two on right) below the cloacal opening. Tongue broad, suboval, not notched posteriorly, fully attached to mouth floor. Pouch opening V-shaped with anterior border at level of posterior edge of sacrum.
SVL: 69.7, TIBL: 41.1, FL: 37.7, HL: 23.3, HW: 27.2, IOD: 9.7, EW: 5.6, IND: 3.9, ED: 5.8, EN: 7.2, TD: 3.9, FFL: 14.5, TFL: 26.8, TFD: 4.2.
In life: dorsal surfaces green with numerous black flecks on dorsum, chocolate brown labial stripe, groins and ventrolateral region yellowish brown; forelimbs and fingers blotched with chocolate brown; hindlimbs with scattered black irregular flecks, numerous on shanks, larger on anterior surface of thighs, tarsus and feet; a chocolate brown stripe from the middle of thighs, running along knee, inner edge of shank and outer edge tarsus to toe V; toes chocolate brown. Ventral surface of throat, chest, forelimbs, belly and thighs yellowish green with a large greyish brown patch on the middle of the belly and a dark greyish brown patch on thighs; tibia and shanks green, ventral surface of tarsus, palms and soles dark greyish brown. Iris silvery with a light blue hue. In preservative (ethanol 70%): green coloration turns light blue and chocolate brown coloration turns pale brown on dorsal surface; ventrally, yellowish green coloration turns greyish light blue and dark greyish brown coloration turns light brown.
Morphometric variation of two males and two females is summarized in Table
The dorsum in males (
The froglets (
Three living adult specimens of Gastrotheca gemma sp. nov.: (A–B) dorsolateral and ventral views of female holotype (
Gastrotheca gemma | ||||
21246♂ | 21247♂ | 21238♀ | 19396♀ | |
SVL | 57.0 | 59.5 | 69.7 | 71.8 |
TIBL | 33.4 | 34.2 | 41.1 | 39.7 |
FL | 31.8 | 30.5 | 37.7 | 35.6 |
HL | 19.5 | 19.7 | 23.3 | 24.8 |
HW | 24.2 | 24.6 | 27.2 | 27.8 |
IOD | 8.0 | 8.8 | 9.7 | 10.1 |
EW | 5.0 | 5.0 | 5.6 | 5.2 |
IND | 3.7 | 3.7 | 3.9 | 4.1 |
ED | 5.4 | 5.1 | 5.8 | 5.4 |
EN | 5.4 | 5.9 | 7.2 | 6.7 |
TD | 2.4 | 2.5 | 3.9 | 3.0 |
FFL | 11.9 | 12.1 | 14.5 | 15.1 |
TFL | 22.3 | 20.6 | 26.8 | 26.7 |
TFD | 3.5 | 3.2 | 4.2 | 4.2 |
Osteological description of a brooding female paratype of Gastrotheca gemma (
The skull of Gastrotheca gemma is wider than long and measures 22.7 mm in length from the jaw joint to the tip of the snout and 27.0 mm in width at the level of the quadratojugal. The skull is hyperossified, with well-developed pit-and-ridge dermal sculpturing (i.e., exostosis) on the frontoparietals, squamosals, maxillae, and nasals (Fig.
Vertebral column. Eight presacral vertebrae, the low neural spines of the atlas and presacral II articulate while the remaining presacrals are non-imbricate (Fig.
Arciferal pectoral girdle (Fig.
In dorsal view, the ilial shafts have a V–shaped configuration. The ilial shaft has a low dorsal crest (Fig.
The humerus has a prominent ventral crest, extending along more than half the length of the bone, higher at the proximal end of the humerus and gradually diminishing in height distally. The distal head (eminentia capitata) is expanded and it is wider than the glenoid head (caput humeri). The radioulna is flattened and distinctly wide distally; the sulcus intermedius is indicated by a distinct groove on the distal half of the bone. The carpus is composed by radiale, ulnare, distal carpal 5–4–3, element Y fused to distal carpal 2, and elements of the prepollex. The phalangeal formula is 2–2–3–3 (Fig.
The femur is slightly sigmoid, shorter than the tibiofibula. The sulcus intermedius of the tibiofibula is shallow. The tibiale and fibulare are separated medially and fused at the proximal and distal ends. These bones are approximately half the length of the tibiofibula. There are four tarsal elements: element Y, two distal tarsals, and the prehallux. The element Y articulates with the prehallux. There is a single ossified element of the small prehallux. The phalangeal formula is 2–2–3–4–3 (Fig.
Gastrotheca gemma is only known from three close localities on the summit of the Cordillera de Colán, at elevations from 3130 to 3180 m a.s.l. (Fig.
Syntopic species include G. abdita, Pristimantis atrabracus, P. corrugatus, P. sp. and Ctenophryne sp. Three completely developed froglets emerged from the holotype’s dorsal pouch during the photographic session. All froglets were similar in size (SVL between 11.30 and 11.33 mm). One paratype (
We did not detect infection by the fungal pathogen Batrachochytrium dendrobatidis (Bd) in the three sampled specimens of G. gemma (
The specific epithet comes from the Latin word “gemma”, a substantive meaning precious stone or gem. This specific name is used in apposition and refers to the turquoise coloration, in life, of the eyes of the new species of marsupial frog, which resembles the coloration of a turquoise stone.
The description of Gastrotheca gemma increases the number of Gastrotheca species reported from Peru to 31, among these 26 are endemic. Gastrotheca gemma is the second species of the genus described from Cordillera de Colán. The LSU ornithological expedition collected G. abdita, also a direct-developer, more than 40 years ago. This poorly known species was considered the only Gastrotheca species inhabiting Cordillera de Colán.
The cranial morphology of Gastrotheca is morphologically complex, particularly in the Andean clade Gastrotheca marsupiata species group. Gastrotheca galeata possesses a highly derived, casqued skull that bears little similarity to other species in the genus due to a unique sculpturing pattern and extensive synostosis (
The herpetofauna of the isolated Cordillera de Colán deserves further investigation, in fact the expedition in which G. gemma was discovered is one of three expeditions targeting exclusively the unknown diversity of this group.
This research was made possible with the support of the Critical Ecosystem Partnership Fund (CEPF) (project number CEPF–109938) through the Fondo de Promoción de las Áreas Naturales Protegidas del Perú (PROFONANPE). We also thank the Global Genome Initiative (GGBN–GGI) for their support. We are especially grateful with the Servicio Nacional de Áreas Naturales Protegidas por el Estado (SERNANP), especially with the professional personnel of the Santuario Nacional Cordillera de Colán: Christian Olivera, Jhonny D. Ramos, Gerlys Fernandez, and Abner García for its logistic support. We also thank Jesus Ormeño and Santiago Bullard for the company and field assistance.
Specimens collected for this study are covered by the following research permits (issued by the Ministerio de Agricultura and Servicio Nacional de Áreas Naturales y Protegidas por el Estado): 067–2019–MINAGRI–SERFOR–DGGSPFFS and N° 004–2019–SERNANP–JEF. LYE is funded by a scholarship (number 88887.179352/2018-00) from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil and by a grant from The Society of Systematic Biologists (SSB) Graduate Student Research Awards (2018). LYE is also grateful with Adolpho Herbert Augustin and Miriam Vianna from the Instituto do Petróleo e dos Recursos Naturais (IPR). DJP was supported by a NSF Graduate Research Fellowship under Grants DGE-1315138 and DGE-1842473.
Examined specimens
Gastrotheca aguaruna — PERU: Amazonas: Luya: ACP Huiquilla, 2935 m a.s.l.,
Gastrotheca monticola — PERU: Amazonas: Bongara: Valera: Cocachimba, 1620 m a.s.l.,
Gastrotheca sp. — PERU: Amazonas: Rodríguez de Mendoza: Vista Alegre, 3308 m a.s.l.,
Gastrotheca oresbios — PERU: Amazonas: Chachapoyas: Abra Barro Negro, 3290 m a.s.l.,
Gastrotheca spectabilis — PERU: Amazonas: Rodríguez de Mendoza: Vista Alegre: Hornillo, 3308 m a.s.l.,
Species | 12S | 16S | ND1 | POMC | RAG1 (1) |
---|---|---|---|---|---|
Gastrotheca aguaruna KU 212026 | — | KF723438 | KF723462 | KF723484 | KF723505 |
Gastrotheca albolineata MNRJ 54401 | KR559919 | KR270407 + KR270425 | KC844949 | KR270365 | KR138423 |
Gastrotheca antoniiochoai MUSM 27944 | — | JN157622 | KC844950 | KC844972 | KC844993 |
Gastrotheca aratia KU 212056 | — | KF723443 | KF723467 | KF723489 | KF723510 |
Gastrotheca argenteovirens KU 181168 | DQ679233 | DQ679383 | DQ679342 | DQ679311 | — |
Gastrotheca atympana MHNSM 18692 | DQ679234 | DQ679384 | DQ679343 | DQ679312 | DQ679276 |
Gastrotheca aureomaculata KU 181194 | DQ679235 | DQ679385 | DQ679344 | — | DQ679277 |
Gastrotheca christiani FML 2881 | DQ679236 | DQ679386 | DQ679345 | DQ679313 | DQ679278 |
Gastrotheca chrysosticta LM 58 | DQ679237 | DQ679387 | DQ679346 | — | DQ679279 |
Gastrotheca cornuta USNM 572472 + AMNH 107251 | AY843591 | AY843591 | DQ679347 | DQ679314 | DQ679280 |
Gastrotheca cuencana QCAZ 42831 | MG948913 | MG948924 | MH223474 | MH223466 | — |
Gastrotheca dendronastes KU 181203 | DQ679239 | DQ679389 | DQ679348 | DQ679315 | DQ679281 |
Gastrotheca dissimilis KU 181740 | DQ679253 | DQ679402 | DQ679361 | — | — |
Gastrotheca dunni ICN 10059 + MHUA A 4800 | DQ679240 | KR270426 | DQ679349 | DQ679316 | DQ679282 |
Gastrotheca ernestoi MNRJ 57129 + MNRJ 64000 | KR559920 | KR270408 + KR270427 | KC844952 | KR270366 | KR138424 |
Gastrotheca espeletia KU203440 | KJ489465 | KJ489514 | KJ489555 | ||
Gastrotheca excubitor MUSM 26280 | — | JN157623 | — | — | — |
Gastrotheca fissipes ZUFRJ 7901 | — | — | JX262925 | — | — |
Gastrotheca fulvorufa CTMZ 07467 | — | KC844929 | KC844954 | KC844977 | KC844997 |
Gastrotheca galeata KU 181700 | DQ679242 | DQ679392 | DQ679351 | DQ679318 | DQ679284 |
Gastrotheca gracilis DCC 006 | DQ679243 | — | — | DQ679319 | — |
Gastrotheca griswoldi MHNSM 20588 | AM039716 | AM039648 | — | — | — |
Gastrotheca guentheri KU 173112 | DQ679245 | DQ679393 | DQ679353 | DQ679321 | DQ679285 |
Gastrotheca helenae KU 181070 | DQ679246 | DQ679394 | DQ679354 | DQ679322 | DQ679286 |
Gastrotheca pseustes 1 QCAZ 45113 | — | KC844923 | KC844948 | KC844970 | — |
Gastrotheca pseustes 2 QCAZ 42862 + TNHC 62492 | AY326051 | JX564866 | KC844962 | KC844986 | KX208740 |
Gastrotheca litonedis KU 202690 | DQ679247 | DQ679395 | DQ679355 | DQ679323 | DQ679287 |
Gastrotheca lojana QCAZ 42725 + KU 203546 | — | KC844938 | KC844964 | KC844988 | KJ489595 |
Gastrotheca longipes USNM 258905 | DQ679248 | DQ679396 | DQ679356 | DQ679324 | DQ679288 |
Gastrotheca marsupiata KU 214813 + KU 214814 | AY819356 | DQ679397 | AY819487 | AY819105 | DQ679289 |
Gastrotheca megacephala JLG 90 + CFBH T377 | AY843592 | AY843592 | KC844953 | KC844976 | AY844381 + KC844996 |
Gastrotheca microdiscus CFBH T 1250 + CFBH T 3068 | — | KC844932 | KC844958 | KC844979 | KC844999 |
Gastrotheca monticola KU 212036 | AY819357 | DQ679398 | AY819488 | AY819106 | DQ679290 |
Gastrotheca nebulanastes MUSM 27943 + MCZ 265218 | — | JN157625 | KC844959 | KC844982 | KC845001 |
Gastrotheca nicefori KU 181071 | DQ679249 | DQ679399 | DQ679357 | DQ679325 | DQ679291 |
Gastrotheca ochoai KU173499 | DQ679250 | DQ679400 | DQ679358 | DQ679326 | DQ679292 |
Gastrotheca oresbios |
— | KJ489461 | KJ489509 | KJ489552 | KJ489588 |
Gastrotheca orophylax KU 178568 | DQ679251 | DQ679401 | DQ679359 | DQ679327 | DQ679293 |
Gastrotheca ovifera KU 185758 | DQ679252 | — | DQ679360 | — | — |
Gastrotheca pachachacae MUSM 28492 | — | JN157620 | — | KC844983 | KC845002 |
Gastrotheca peruana KU 207815 | KF723451 | KF723475 | KF723497 | — | |
Gastrotheca phalarosa |
— | KJ489459 | KJ489507 | KJ489551 | KJ489585 |
Gastrotheca phelloderma MUSM 33350 | — | MH756004 | — | — | — |
Gastrotheca plumbea KU 178499 | DQ679254 | DQ679403 | DQ679362 | DQ679328 | DQ679294 |
Gastrotheca prasina MZUSP 147060 + MZUSP 17460 | JX262891 | KJ489476 | JX262922 | — | KJ489602 |
Gastrotheca psychrophila KU 142634 | DQ679255 | DQ679404 | DQ679363 | DQ679329 | DQ679295 |
Gastrotheca rebeccae |
— | KC844937 | KC844963 | KC844987 | — |
Gastrotheca recava MZUSP 147044 + MZUSP 147042 | JX262890 | KJ489497 | JX262921 | — | KJ489604 |
Gastrotheca riobambae KU 178468 + KU 203516 | DQ679256 | DQ679405 | DQ679364 | KJ489580 | DQ679296 |
Gastrotheca ruizi KU 200002 | DQ679257 | DQ679406 | DQ679365 | — | DQ679297 |
Gastrotheca yacuri QCAZ 21105 | — | KC844939 | KC844965 | KC844989 | — |
Gastrotheca turnerorum QCAZ 47299 | — | KC844934 | KC844960 | KC844984 | — |
Gastrotheca elicioi QCAZ 21213 | — | KC844922 | KC844947 | — | — |
Gastrotheca spectabilis |
— | KJ489464 | KJ489513 | KJ489554 | KJ489592 |
Gastrotheca stictopleura MTD 45230 | DQ679258 | DQ679407 | DQ679366 | DQ679330 | DQ679298 |
Gastrotheca testudinea QCAZ 16444 | — | KC844940 | KC844966 | — | — |
Gastrotheca trachyceps KU 181189 | DQ679259 | DQ679408 | DQ679367 | DQ679331 | DQ679299 |
Gastrotheca walkeri Vz 8996 | DQ679260 | DQ679409 | DQ679368 | DQ679332 | DQ679300 |
Gastrotheca weinlandii KU 143105 | DQ679261 | DQ679410 | DQ679369 | DQ679333 | DQ679301 |
Gastrotheca zeugocystis MHNSM 18675 | DQ679262 | DQ679411 | — | DQ679334 | DQ679302 |
Gastrotheca sp. 1 IDLR 4073 (MNCN/ADN 566) | — | KR270428 | — | — | — |
Gastrotheca sp. 2 MNK 5286 + CBG 1020 | AY843590 | AY843590 | KC844955 | — | AY844380 |
Gastrotheca sp. 3 ZFMK 66954 | — | KR270429 | — | — | — |
Gastrotheca sp. J |
— | KJ489463 | KJ489511 | — | KJ489590 |
Gastrotheca sp. |
— | KJ489475 | KJ489525 | KJ489563 | — |
Gastrotheca sp. KU 173171 | DQ679241 | DQ679391 | DQ679350 | DQ679317 | DQ679283 |
Gastrotheca gemma |
— | MW403923 | — | — | — |
Hemiphractus proboscideus | AY819358 | DQ679413 | AY819489 | AY819107 | DQ679304 |