Maximum likelihood optimal tree (log likelihood = –36764.630331) inferred from 4,919 bp of mitochondrial (12S rRNA, 16S rRNA, ND1) and nuclear (POMC and RAG1) gene sequences. The phylogenetic tree depicts the relationships of Gastrotheca gemma sp. nov. with 67 Gastrotheca terminals, including 59 described species. Bootstrap support values of 100% are represented by an asterisk. Species groups are abbreviated as s.g.